2QXZ
pectate lyase R236F from Xanthomonas campestris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-04-19 |
| Detector | RIGAKU RAXIS HTC |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.329, 53.169, 73.218 |
| Unit cell angles | 71.90, 80.23, 69.11 |
Refinement procedure
| Resolution | 69.500 - 2.120 |
| R-factor | 0.14954 |
| Rwork | 0.147 |
| R-free | 0.19352 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qx3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.180 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.500 | |
| High resolution limit [Å] | 2.120 | 2.120 |
| Number of reflections | 32855 | |
| Completeness [%] | 85.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 25% PEG 3350, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
