2QXL
Crystal Structure Analysis of Sse1, a yeast Hsp110
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97923 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 218.045, 125.331, 74.708 |
Unit cell angles | 90.00, 98.67, 90.00 |
Refinement procedure
Resolution | 29.490 - 2.410 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.23500 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.193 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.08) |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.160 | 2.400 |
Rmerge | 0.055 | 0.039 | 0.279 |
Number of reflections | 75393 | ||
<I/σ(I)> | 15.9 | ||
Completeness [%] | 99.6 | 98.2 | 99.7 |
Redundancy | 3.6 | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10% PEG 6000, 0.1M cacodylate, pH 6.5, temperature 293K, VAPOR DIFFUSION, HANGING DROP |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10% PEG 6000, 0.1M cacodylate, pH 6.5, temperature 293K, VAPOR DIFFUSION, HANGING DROP |