2QXF
Product bound structure of exonuclease I at 1.5 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-26 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.630, 91.849, 102.752 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.320 - 1.500 |
R-factor | 0.20044 |
Rwork | 0.199 |
R-free | 0.22357 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.759 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR (2.5) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
Rmerge | 0.040 | 0.022 | 0.371 |
Number of reflections | 80289 | ||
<I/σ(I)> | 16.2 | ||
Completeness [%] | 99.8 | 98.6 | 99.7 |
Redundancy | 6.8 | 6.8 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.2M Sodium chloride, 0.1M Tris-HCl, 20% PEG 8000, 1.5x molar excess ssDNA, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |