2QX9
Crystal Structure of Quinone Reductase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.025, 81.528, 106.178 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.310 |
| Rwork | 0.213 |
| R-free | 0.28600 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.427 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.320 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.203 | 0.888 |
| Number of reflections | 22987 | 3619 |
| <I/σ(I)> | 7.1 | 2.3 |
| Completeness [%] | 99.0 | 99.2 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | BisTris, NaCl, Ammonium sulfate, 2-iodomelatonin, DTT, FAD, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
