2QX8
Crystal Structure of Quinone Reductase II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.595, 83.322, 106.213 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
Rwork | 0.211 |
R-free | 0.23500 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.618 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.320 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.091 | 0.787 |
Number of reflections | 136994 | 20482 |
<I/σ(I)> | 13.85 | 1.5 |
Completeness [%] | 98.3 | 92.1 |
Redundancy | 7 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | BisTris, NaCl, Ammonium Sulfate, FAD, DTT, 2-iodomelatonin, Glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |