2QX8
Crystal Structure of Quinone Reductase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.595, 83.322, 106.213 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| Rwork | 0.211 |
| R-free | 0.23500 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.618 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.320 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.091 | 0.787 |
| Number of reflections | 136994 | 20482 |
| <I/σ(I)> | 13.85 | 1.5 |
| Completeness [%] | 98.3 | 92.1 |
| Redundancy | 7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | BisTris, NaCl, Ammonium Sulfate, FAD, DTT, 2-iodomelatonin, Glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
