2QX5
Structure of nucleoporin Nic96
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0718 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.121, 82.478, 113.028 |
| Unit cell angles | 90.00, 91.11, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| Rwork | 0.233 |
| R-free | 0.28500 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.712 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 55097 | |
| <I/σ(I)> | 19.9 | 3.7 |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 289 | 18% PEG 3350, 0.1M Bis Tris propane, 0.2M Potassium Thiocyanate, 1mM Cetyltrimethylammonium bromide, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
