2QWU
Crystal structure of F. tularensis pathogenicity island protein C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97939 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.031, 83.991, 89.838 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.650 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.22000 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SGXPRO |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.080 | 0.445 |
| Number of reflections | 78172 | |
| <I/σ(I)> | 17.6 | 2.9 |
| Completeness [%] | 96.8 | 78.6 |
| Redundancy | 6.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 100mM Tris-HCl pH 8.5, 27-30% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
