2QWT
Crystal structure of the TetR transcription regulatory protein from Mycobacterium vanbaalenii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.561, 57.561, 98.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.256 |
| Rwork | 0.255 |
| R-free | 0.27900 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.679 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.690 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.099 | 0.460 |
| Number of reflections | 7823 | |
| <I/σ(I)> | 37.8 | 6.6 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 26.8 | 27.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 400mM Potassium sodium tartrate, pH 7.0, VAPOR DIFFUSION, temperature 294K |
