2QWM
Crystal structure of bovine hsc70 (1-394aa)in the ADP*Vi state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-29 |
| Detector | RIGAKU RAXIS HTC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.336, 77.841, 75.793 |
| Unit cell angles | 90.00, 101.53, 90.00 |
Refinement procedure
| Resolution | 36.060 - 1.860 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.173 |
| Data reduction software | DENZO |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 4.010 | 1.860 |
| Rmerge | 0.061 | 0.020 | 0.415 |
| Number of reflections | 70267 | ||
| <I/σ(I)> | 13.4 | ||
| Completeness [%] | 100.0 | 99.9 | 99.8 |
| Redundancy | 3.7 | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 8 | 289 | PEG3350, Calcium Acetate, pH 8.0, microbatch under oil, temperature 289K |
