2QW8
Structure of Eugenol Synthase from Ocimum basilicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.799, 85.935, 76.153 |
| Unit cell angles | 90.00, 107.27, 90.00 |
Refinement procedure
| Resolution | 500.000 - 1.600 |
| Rwork | 0.205 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.290 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.660 | |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.083 | 0.676 |
| Number of reflections | 84783 | 8699 |
| <I/σ(I)> | 9.92 | 2.4 |
| Completeness [%] | 97.2 | 95.9 |
| Redundancy | 3.74 | 3.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1 M MOPSO, 28% PEG MME5000, 0.3 M KNO3, 2 mM dithiothreitol, 5 mM NADP+, pH 7.0, vapor diffusion, hanging drop, temperature 277K |
