2QW1
Glucose/galactose binding protein bound to 3-O-methyl D-glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-25 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.930, 74.660, 110.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.700 |
R-factor | 0.18159 |
Rwork | 0.180 |
R-free | 0.20426 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fw0 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.295 |
Data reduction software | SAINT |
Data scaling software | PROTEUM PLUS (PLUS) |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.750 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 47220 | |
<I/σ(I)> | 25.15 | 3.82 |
Completeness [%] | 90.1 | 56.8 |
Redundancy | 6.31 | 2.11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 2.0 M ammonium sulfate and 0.05 M sodium citrate dehydrate, 3-O-methyl glucose (5mM) was soaked in. 2.5 M Na Malonate cryoprotectant, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |