2QW1
Glucose/galactose binding protein bound to 3-O-methyl D-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-25 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.930, 74.660, 110.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.700 |
| R-factor | 0.18159 |
| Rwork | 0.180 |
| R-free | 0.20426 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fw0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.295 |
| Data reduction software | SAINT |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 47220 | |
| <I/σ(I)> | 25.15 | 3.82 |
| Completeness [%] | 90.1 | 56.8 |
| Redundancy | 6.31 | 2.11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 2.0 M ammonium sulfate and 0.05 M sodium citrate dehydrate, 3-O-methyl glucose (5mM) was soaked in. 2.5 M Na Malonate cryoprotectant, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
