2QVM
The second Ca2+-binding domain of the Na+-Ca2+ exchanger is essential for regulation: crystal structures and mutational analysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.030, 59.232, 22.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.800 - 1.703 |
| R-factor | 0.18635 |
| Rwork | 0.185 |
| R-free | 0.21926 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qvk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.448 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 1.760 |
| High resolution limit [Å] | 1.703 | 1.703 |
| Rmerge | 0.069 | 0.489 |
| Number of reflections | 12622 | |
| <I/σ(I)> | 25.4 | 3 |
| Completeness [%] | 99.8 | 98.2 |
| Redundancy | 12.8 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | 21% (w/v) PEG-3350, 0.1 M HEPES (pH 6.3), CBD2 at 15 mg/ml, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
