2QVG
The crystal structure of a two-component response regulator from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 49.797, 49.797, 111.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.200 - 1.500 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.25500 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.047 | 0.156 |
| Number of reflections | 44110 | |
| <I/σ(I)> | 21.7 | 7 |
| Completeness [%] | 89.1 | 45.6 |
| Redundancy | 9.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 0.2M Lithium sulfate, 0.1M Bis-Tris pH 5.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
