2QVC
Crystal structure of a periplasmic sugar ABC transporter from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | H 3 |
| Unit cell lengths | 123.325, 123.325, 280.593 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.830 - 2.400 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.096 | 0.390 |
| Number of reflections | 60160 | |
| <I/σ(I)> | 13 | 2.5 |
| Completeness [%] | 96.0 | 72.3 |
| Redundancy | 9.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 30% PEG 4000, 0.2 M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
