2QV8
Structure of the minor pseudopilin EpsH from the Type 2 Secretion System of Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-16 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97903 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.349, 70.449, 85.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.171 |
Rwork | 0.169 |
R-free | 0.21200 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.537 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.060 |
High resolution limit [Å] | 1.990 | 4.290 | 1.990 |
Rmerge | 0.070 | 0.043 | 0.271 |
Number of reflections | 21140 | ||
<I/σ(I)> | 11.5 | ||
Completeness [%] | 93.2 | 99.9 | 100 |
Redundancy | 7.1 | 6.8 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.5 | 298 | PEG 4000, pH 4.5, vapor diffusion, temperature 298K |