2QV2
A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2005-07-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793, 0.9840 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 91.300, 91.300, 103.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.420 - 2.400 |
| Rwork | 0.248 |
| R-free | 0.28800 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.240 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.420 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.062 |
| Number of reflections | 18866 |
| <I/σ(I)> | 43.9 |
| Completeness [%] | 94.3 |
| Redundancy | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 100 mM Hepes, 8.5% PEG8000, 400 mM NaCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
