2QUD
PP7 Coat Protein Dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.008 |
| Spacegroup name | H 3 |
| Unit cell lengths | 154.985, 154.985, 31.631 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.750 - 1.600 |
| R-factor | 0.159 |
| Rwork | 0.157 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dwn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.512 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.032 | 0.475 |
| Number of reflections | 37546 | |
| <I/σ(I)> | 36.8 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 1.1M Na Malonat, 0.1M HEPES, 0.5% Jeffamine ED-2001, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
