2QUB
Crystal structure of extracellular lipase LipA from Serratia marcescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 202.401, 202.401, 317.731 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.930 - 1.800 |
| R-factor | 0.171 |
| Rwork | 0.171 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.098 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0036) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.066 | 0.335 |
| Number of reflections | 430068 | |
| <I/σ(I)> | 11.6 | 2.6 |
| Completeness [%] | 95.6 | 76.7 |
| Redundancy | 24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 281 | 26% MPD, 0.1M Na-Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 281K, pH 4.60 |
