2QU7
Crystal structure of a putative transcription regulator from Staphylococcus saprophyticus subsp. saprophyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 93.417, 116.382, 68.201 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.208 |
| Rwork | 0.205 |
| R-free | 0.26800 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.318 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.932 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.158 | 0.819 |
| Total number of observations | 38941 | |
| Number of reflections | 33797 | |
| <I/σ(I)> | 11.5 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.9 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 294 | 200 mM Sodium acetate pH 4.6, 100mM Tris-HCl pH 8.5, 30% PEG 4000, VAPOR DIFFUSION, temperature 294K |
