2QTX
Crystal structure of an Hfq-like protein from Methanococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.866, 67.110, 119.064 |
| Unit cell angles | 90.00, 104.03, 90.00 |
Refinement procedure
| Resolution | 38.500 - 2.500 |
| Rwork | 0.180 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1s |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.928 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 250.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.056 | 0.209 |
| Number of reflections | 28424 | |
| <I/σ(I)> | 17 | 3.7 |
| Completeness [%] | 92.5 | 57.5 |
| Redundancy | 3.4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 277 | 25% PEG 3350, 0.2M Ammonium Acetate, 0.1M Tris, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 8.50 |
