2QTT

Crystal Structure of Arabidopsis thaliana 5'-Methylthioadenosine nucleosidase in complex with Formycin A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-06-30
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	41.300, 127.050, 45.450
Unit cell angles	90.00, 109.63, 90.00

Refinement procedure

Resolution	23.610 - 1.930
R-factor	0.17771
Rwork	0.172
R-free	0.23275
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.509
Data reduction software	d*TREK
Data scaling software	d*TREK (9.4L)
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.610	2.000
High resolution limit [Å]	1.930	1.930
Rmerge	0.060	0.155
Number of reflections	31340
<I/σ(I)>	12.6	5.2
Completeness [%]	94.8	90.5
Redundancy	3.17	2.48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	295	NH4I, PEG3350, ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 7.50
1		7.5	295	NH4I, PEG3350, ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 7.50