2QTP

Crystal structure of a duf1185 family protein (spo0826) from silicibacter pomeroyi dss-3 at 2.10 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-04
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162, 0.97922, 0.97905
Spacegroup name	P 43 21 2
Unit cell lengths	94.730, 94.730, 47.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.308 - 2.100
R-factor	0.207
Rwork	0.204
R-free	0.25900
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.682
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.308	27.308	2.170
High resolution limit [Å]	2.100	4.510	2.100
Rmerge	0.038	0.022	0.717
Number of reflections	13007	2361	2199
<I/σ(I)>	15.82	45.2	2
Completeness [%]	99.3	96.1	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	NANODROP, 0.1M Sodium dihydrogen phosphate, 0.1M Potassium dihydrogen phosphate, 2.0M Sodium chloride, 0.1M MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K