2QTO
An anisotropic model for potassium channel KcsA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.908 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.780, 68.930, 112.040 |
| Unit cell angles | 90.00, 124.63, 90.00 |
Refinement procedure
| Resolution | 9.990 - 3.201 |
| R-factor | 0.27164 |
| Rwork | 0.272 |
| R-free | 0.27181 |
| Structure solution method | MIR |
| Starting model (for MR) | 1bl8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.989 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.201 |
| Number of reflections | 12603 |
| Completeness [%] | 93.3 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 200 MM CACL2, 100 MM HEPES PH 7.5, 48% PEG 400 |
