2QTO
An anisotropic model for potassium channel KcsA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Wavelength(s) | 0.908 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 128.780, 68.930, 112.040 |
Unit cell angles | 90.00, 124.63, 90.00 |
Refinement procedure
Resolution | 9.990 - 3.201 |
R-factor | 0.27164 |
Rwork | 0.272 |
R-free | 0.27181 |
Structure solution method | MIR |
Starting model (for MR) | 1bl8 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.989 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 3.201 |
Number of reflections | 12603 |
Completeness [%] | 93.3 |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 200 MM CACL2, 100 MM HEPES PH 7.5, 48% PEG 400 |