2QTG
Crystal Structure of Arabidopsis thaliana 5'-Methylthioadenosine nucleosidase in complex with 5'-methylthiotubercidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.580, 126.050, 45.540 |
| Unit cell angles | 90.00, 109.02, 90.00 |
Refinement procedure
| Resolution | 32.770 - 1.840 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.714 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.4L) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.770 | 1.910 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.060 | 0.299 |
| Number of reflections | 35711 | |
| <I/σ(I)> | 16.2 | 5.7 |
| Completeness [%] | 95.5 | 97.3 |
| Redundancy | 6.36 | 6.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 295 | NH4I, PEG3350, ethylene glycol, pH 7.5, vapor diffusion, hanging drop, temperature 295K, pH 7.50 |
