2QT3

Crystal structure of N-Isopropylammelide isopropylaminohydrolase AtzC from Pseudomonas sp. strain ADP complexed with Zn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	P 41 21 2
Unit cell lengths	102.969, 102.969, 225.558
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.970 - 2.240
R-factor	0.226
Rwork	0.226
R-free	0.24700
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.970
High resolution limit [Å]	2.240
Number of reflections	56960
Completeness [%]	96.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	45% MPD, 0.1 M Bis-Tris, 0.2 M Ammonium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K