2QSX
Crystal structure of putative transcriptional regulator LysR From Vibrio parahaemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.291, 83.110, 95.509 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.750 - 1.640 |
| R-factor | 0.20625 |
| Rwork | 0.204 |
| R-free | 0.23982 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.526 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.750 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.070 | 0.390 |
| Number of reflections | 45578 | |
| <I/σ(I)> | 31.9 | 3.7 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 8.6 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1 M MES Na pH 6.5, 0.8 M Magnesium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
