2QSW
Crystal structure of C-terminal domain of ABC transporter / ATP-binding protein from Enterococcus faecalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-30 |
Detector | SBC-3 |
Wavelength(s) | 0.97920 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 38.896, 38.896, 111.938 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.800 - 1.500 |
R-factor | 0.149 |
Rwork | 0.147 |
R-free | 0.18420 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.704 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.800 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.067 | 0.438 |
Number of reflections | 14492 | |
<I/σ(I)> | 45.5 | 2.48 |
Completeness [%] | 99.4 | 94.2 |
Redundancy | 11.2 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 277 | 0.01 M Zinc sulfate, 0.1 M MES buffer pH 6.9, 25% PEG 550 MME, VAPOR DIFFUSION, SITTING DROP, temperature 277K |