2QSK
Atomic-resolution crystal structure of the Recombinant form of Scytovirin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.200, 38.790, 59.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.000 |
| R-factor | 0.129 |
| Rwork | 0.128 |
| R-free | 0.14700 |
| Structure solution method | SAD/DIRECT REFINEMENT |
| Starting model (for MR) | 2qt4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.392 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.057 | 0.404 |
| Number of reflections | 48018 | |
| <I/σ(I)> | 19.3 | 1.9 |
| Completeness [%] | 98.8 | 85.3 |
| Redundancy | 4.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1M sodium citrate, 0.1 M imidazole pH 8.0, protein concentration 32 MG/ML VAPOR DIFFUSION, HANGING DROP, temperature 293K |
