2QSJ
Crystal structure of a LuxR family DNA-binding response regulator from Silicibacter pomeroyi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-29 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 41 |
| Unit cell lengths | 57.459, 57.459, 111.354 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.26700 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.554 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.459 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.066 | 0.592 |
| Number of reflections | 21057 | |
| <I/σ(I)> | 24.9 | 4.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 12.6 | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 294 | 100 mM Bis-Tris pH 5.5, 17% PEG 10000, 100 mM Ammonium acetate, VAPOR DIFFUSION, temperature 294K |
