2QSI
Crystal structure of putative hydrogenase expression/formation protein hupG from Rhodopseudomonas palustris CGA009
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97940 |
Spacegroup name | P 1 |
Unit cell lengths | 31.575, 41.126, 46.850 |
Unit cell angles | 107.72, 99.85, 101.75 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.15455 |
Rwork | 0.152 |
R-free | 0.20133 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.058 | 0.124 |
Number of reflections | 17173 | |
<I/σ(I)> | 14.4 | 7.8 |
Completeness [%] | 87.2 | |
Redundancy | 2.7 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 1.5M Ammonium sulfate, 50mM MES pH 5.6, 2% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 291K |