2QSC
Crystal structure analysis of anti-HIV-1 V3-Fab F425-B4e8 in complex with a V3-peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-01 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 82.912, 39.858, 97.960 |
Unit cell angles | 90.00, 108.50, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.800 |
R-factor | 0.222 |
Rwork | 0.219 |
R-free | 0.26400 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.946 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 15169 | |
<I/σ(I)> | 12.6 | 2.3 |
Completeness [%] | 99.0 | 94.6 |
Redundancy | 3.7 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 298 | PEG 8000, pH 6.0, VAPOR DIFFUSION, temperature 298K |