2QSA
Crystal structure of J-domain of DnaJ homolog dnj-2 precursor from C.elegans.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | H 3 |
| Unit cell lengths | 81.148, 81.148, 60.016 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.700 - 1.680 |
| R-factor | 0.1639 |
| Rwork | 0.163 |
| R-free | 0.18030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2O37 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.462 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.700 | 1.720 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.045 | 0.479 |
| Number of reflections | 16389 | |
| <I/σ(I)> | 33.7 | 1.94 |
| Completeness [%] | 97.5 | 74.8 |
| Redundancy | 5.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 287 | 2.5 M NaCl, 0.1 M Tris buffer pH 7.0, 0.2 M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 287K |
