2QS4
Crystal structure of the GluR5 ligand binding core dimer in complex with LY466195 at 1.58 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 89.241, 89.241, 330.365 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.640 - 1.580 |
| R-factor | 0.16026 |
| Rwork | 0.158 |
| R-free | 0.19948 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f34 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.614 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.059 | 0.445 |
| Number of reflections | 134309 | |
| <I/σ(I)> | 14.6 | 3.06 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.35 | 293 | 20% PEG 3350, 250mM Ammonium Citrate, 2.5mM LY466195, pH 5.35, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
