2QS2
Crystal structure of the GluR5 ligand binding core dimer in complex with UBP318 at 1.80 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.91915 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 98.124, 98.176, 128.594 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.290 - 1.800 |
| R-factor | 0.19264 |
| Rwork | 0.191 |
| R-free | 0.21869 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2f34 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.179 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.338 |
| Number of reflections | 58102 | |
| <I/σ(I)> | 14.2 | 5.22 |
| Completeness [%] | 93.7 | 96.2 |
| Redundancy | 7.4 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 285 | 21% PEG 1K, 100mM Tris-Cl, 2.5mM UBP318, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
