2QRO
Human Deoxycytidine kinase dAMP, UDP, Mg ion product complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 93.688, 93.688, 332.408 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.600 - 3.450 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1P60.pdb |
RMSD bond length | 0.009 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.600 | 3.670 |
High resolution limit [Å] | 3.450 | 3.400 |
Rmerge | 0.105 | |
Number of reflections | 17685 | |
<I/σ(I)> | 13.6 | 3.2 |
Completeness [%] | 89.1 | 89 |
Redundancy | 8.3 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 16-17% ethanol, 8% glycerol, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |