2QRN
Human Deoxycytidine kinase dCMP, UDP, Mg ion product complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9764 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 94.565, 94.565, 335.334 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.120 - 3.400 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1P60.pdb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.120 | 3.610 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.129 | |
| Number of reflections | 19703 | |
| <I/σ(I)> | 10.2 | 2.5 |
| Completeness [%] | 95.4 | 73.3 |
| Redundancy | 5.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 16-17% ethanol,0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
