2QRC
Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with ADP and AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97926 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 168.293, 78.087, 108.553 |
Unit cell angles | 90.00, 124.13, 90.00 |
Refinement procedure
Resolution | 48.280 - 2.700 |
R-factor | 0.2106 |
Rwork | 0.206 |
R-free | 0.28945 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2ooy |
RMSD bond length | 0.015 |
RMSD bond angle | 1.609 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.107 | 0.472 |
Number of reflections | 30918 | |
<I/σ(I)> | 7.4 | 1.5 |
Completeness [%] | 96.8 | 81.6 |
Redundancy | 3.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 6-10% PEG 3350, 0.1M Sodium Citrate, pH 5.5, 5mM ADP, 5mM AMP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |