2QR7
2.0A X-ray structure of C-terminal kinase domain of p90 ribosomal S6 kinase 2: Se-Met derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97936 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 46.585, 46.585, 293.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.2045 |
Rwork | 0.203 |
R-free | 0.23776 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.217 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.060 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.079 | 0.440 |
Number of reflections | 23160 | |
<I/σ(I)> | 36.84 | 6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.7 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 7.5%-10% PEG3350, 50mM Ammonium sulfate, 0.1M Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |