2QR4
Crystal structure of oligoendopeptidase-F from Enterococcus faecium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97930 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 133.142, 133.142, 171.619 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.040 - 2.500 |
R-factor | 0.191 |
Rwork | 0.188 |
R-free | 0.24400 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.142 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.590 |
High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
Rmerge | 0.102 | 0.055 | 0.764 |
Number of reflections | 53824 | ||
<I/σ(I)> | 6.4 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 15.8 | 15.1 | 15.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 0.1M Sodium acetate pH 4.6, 1.0M Sodium formate, VAPOR DIFFUSION, SITTING DROP |