2QQZ
Crystal structure of putative glyoxalase family protein from Bacillus anthracis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-03 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97850 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.314, 53.262, 108.115 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.850 - 1.920 |
| R-factor | 0.2 |
| Rwork | 0.196 |
| R-free | 0.23600 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.723 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.850 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 25719 | |
| <I/σ(I)> | 8.6 | 4.4 |
| Completeness [%] | 98.9 | 98.3 |
| Redundancy | 8.1 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2M Ammonium sulfate, 0.1M MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
