2QQV
Crystal structure of a cell-wall invertase (E203A) from Arabidopsis thaliana in complex with sucrose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.81620 |
| Spacegroup name | P 32 |
| Unit cell lengths | 107.131, 107.131, 50.832 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.630 - 3.010 |
| R-factor | 0.20052 |
| Rwork | 0.199 |
| R-free | 0.23698 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ac1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.992 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 11744 | |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M sodium cacodylate, 0.1M zinc acetate, 28% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
