2QQR
JMJD2A hybrid tudor domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97913 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 134.242, 134.242, 134.242 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.870 - 1.800 |
| R-factor | 0.18369 |
| Rwork | 0.182 |
| R-free | 0.21307 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gfa |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.601 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.047 | 0.535 |
| Number of reflections | 35286 | |
| <I/σ(I)> | 57.2 | 4.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.4 | 15.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
