2QQP
Crystal Structure of Authentic Providence Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 659.790, 434.070, 415.850 |
| Unit cell angles | 90.00, 126.13, 90.00 |
Refinement procedure
| Resolution | 49.420 - 3.800 |
| R-factor | 0.285 |
| Rwork | 0.285 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ohf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.158 | 0.249 |
| Number of reflections | 272211 | |
| <I/σ(I)> | 3.6 | 1.9 |
| Completeness [%] | 29.6 | 28.2 |
| Redundancy | 1.3 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 2 ul of 7mg/ml virus solution (20mM Tris pH 7.5) added to 2 ul of 100mM Tris pH 7.5, 0.5-1.5% PEG8000, 10-100mM NaCl. Plates about 0.1 mm in size appear in 1-4 weeks., vapor diffusion, hanging drop, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | 20mM Tris | ||
| 2 | 1 | 2 | 100mM Tris | ||
| 3 | 1 | 2 | PEG8000 | ||
| 4 | 1 | 2 | NaCl |
