2QQK
Neuropilin-2 a1a2b1b2 Domains in Complex with a Semaphorin-Blocking Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.277, 105.873, 92.400 |
| Unit cell angles | 90.00, 98.83, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.750 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2QQJ (b1b2 domains) 2FJH (antibody) 1NT0 (a2 domain) |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.258 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 36321 | |
| <I/σ(I)> | 10.9 | 3.3 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 0.1 M Hepes, 22% polyacrylic acid 5100, 0.02 M MgCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
