2QQ8

Crystal structure of the putative RabGAP domain of human TBC1 domain family member 14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-12
Detector	ADSC QUANTUM 270
Wavelength(s)	0.91770
Spacegroup name	P 43 21 2
Unit cell lengths	123.520, 123.520, 48.608
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.195
R-free	0.23500
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.286
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (5.3.0037)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.098	0.065	0.488
Number of reflections	48699
<I/σ(I)>	7.9
Completeness [%]	100.0	100	99.9
Redundancy	11.4	11.1	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	25% PEG 4000, 0.2M Lithium sulfate, 0.1M Tris-HCl, pH 7.5, VAPOR DIFFUSION, temperature 291K
1	VAPOR DIFFUSION	7.5	291	25% PEG 4000, 0.2M Lithium sulfate, 0.1M Tris-HCl, pH 7.5, VAPOR DIFFUSION, temperature 291K