2QPW
Methyltransferase domain of human PR domain-containing protein 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-24 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97770 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.462, 51.196, 70.887 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.490 - 1.790 |
R-factor | 0.17104 |
Rwork | 0.168 |
R-free | 0.22157 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Obtained via Se-SAD from second crystal (different crystal form) at low resolution |
RMSD bond length | 0.019 |
RMSD bond angle | 1.507 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.790 | 1.790 |
Number of reflections | 23999 | |
<I/σ(I)> | 34.3 | 6 |
Completeness [%] | 99.7 | 99.3 |
Redundancy | 7.2 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 22% PEG 5000 MME, 0.2 M Ammonium sulfate, 0.1 M MES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |