2QPA
Crystal Structure of S.cerevisiae Vps4 in the presence of ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Detector technology | CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.630, 120.640, 157.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.200 |
R-factor | 0.255 |
Rwork | 0.255 |
R-free | 0.30500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.281 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 6.890 | 3.200 |
Rmerge | 0.042 | 0.021 | 0.475 |
Number of reflections | 24259 | ||
<I/σ(I)> | 16.3 | ||
Completeness [%] | 99.4 | 97.3 | 97.3 |
Redundancy | 3.7 | 3.4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.8 | 293 | NaH2PO4/K2HPO4,glycerol, pH 5.8, vapor diffusion, temperature 293K |