2QOQ
Human EphA3 kinase and juxtamembrane region, base, AMP-PNP bound structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-03-23 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.043, 38.324, 76.514 |
Unit cell angles | 90.00, 102.33, 90.00 |
Refinement procedure
Resolution | 27.830 - 1.600 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gsf |
RMSD bond length | 0.008 |
RMSD bond angle | 1.192 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.660 |
High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
Rmerge | 0.052 | 0.053 | 0.175 |
Number of reflections | 37433 | ||
<I/σ(I)> | 30.8 | ||
Completeness [%] | 91.7 | 98.4 | 48.7 |
Redundancy | 6.6 | 6.4 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 20 mg/mL Protein, 25% PEG 3350, 0.2M Ammonium sulfate, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |