2QOP
Crystal structure of the transcriptional regulator AcrR from Escherichia coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979249 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 48.218, 54.574, 73.726 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.550 |
| Rwork | 0.228 |
| R-free | 0.27300 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.076 | 0.306 |
| Number of reflections | 6766 | |
| <I/σ(I)> | 22 | 3.3 |
| Completeness [%] | 98.2 | 85.2 |
| Redundancy | 6.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 35% PEG 4000, 0.2M MgCl2, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
