2QOJ
Coevolution of a homing endonuclease and its host target sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2000-12-09 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.353, 72.701, 61.100 |
Unit cell angles | 90.00, 103.88, 90.00 |
Refinement procedure
Resolution | 29.300 - 2.400 |
R-factor | 0.219 |
Rwork | 0.216 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.753 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.300 | 2.470 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.035 | 0.140 |
Number of reflections | 20093 | |
<I/σ(I)> | 18.4 | 5.7 |
Completeness [%] | 92.1 | 76.8 |
Redundancy | 3.3 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | pH 5 |